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. 2021 Oct 20;13:751913. doi: 10.3389/fnagi.2021.751913

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Copyright © 2021 Shyu, Gao, Wu, He, Cheng and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MAMPH administrations were tested to label c-Fos expression in the NAc and the amygdala’s CeA, and BLA for the control (n = 6), MAMPH (n = 6), PrL(+)/MAMPH (n = 6), and PrL(–)/MAMPH groups (n = 6) by injecting NMDA or saline into the PrL. PrL(+): Injection of a low concentration of NMDA to excite the PrL neurons; PrL(–): Injection of a high concentration of NMDA to destroy the PrL neurons. Differences in c-Fos expression levels in the NAc, CeA, and BLA among all groups were analyzed by one-way ANOVA, followed by Tukey’s post hoc test if a significant difference was appeared among groups. ^∗p < 0.05 indicates significant differences compared with the control group; ^#p < 0.05 indicates significant differences compared with the MAMPH group.