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. 2021 Nov 1;135(21):2467–2481. doi: 10.1042/CS20210612

Figure 1. Knockdown of xbp1s mitigated MCT-induced PH.

Figure 1

Forty SD male rats were randomly assigned to four groups. MCT was administered through intraperitoneal injection, and AAV was administered through intratracheal injection. AAV-xbp1s was designed to knock down the expression of xbp1s in rodents. Rats in the control group were injected with MCT dissolvant, the MCT group was injected with 60 mg/kg MCT, the MCT+AAV-CTL group was injected with MCT and AAV-carried scrambled shRNA (AAV-CTL), and the MCT+AAV-xbp1s group was injected with MCT and AAV-xbp1s. (A) Experimental design and time course of the rodent PH model. (B) Image captured by fluorescence microscopy to trace the location and expression of injected AAV in rodent lung tissues. (C) Representative image of RVSP measurement. Summarized data of RVSP (D), total pulmonary resistance (TPR) (E), right ventricular hypertrophy index (F), systemic blood pressure (SBP) (G), cardiac output (H) and body weight at killing (I). Data are represented as the mean ± SD, n=8–10. ns, no significance, *P<0.05, **P<0.01, ****P<0.0001.