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. 2021 Oct 20;12:757186. doi: 10.3389/fpls.2021.757186

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Copyright © 2021 Forestier, Brown, Harvey, Larson and Graham.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GC–MS total ion chromatograms (TIC) showing the diterpene profile of N. benthamiana infiltrated with casbene synthase (CAS) and different precursor genes. (A) Co-expression of DXS+GGPPS+CAS. (B) Co-expression of DXS+HDR+GGPPS+CAS. (C) Simplified diagram of the MEP pathway plus geranylgeranyl pyrophosphate synthase (GGPPS). MEP pathway enzymes are DXS (1-deoxy-D-xylulose 5-phosphate synthase), DXR (1-deoxy-D-xylulose 5-phosphate reductoisomerase), MCT (2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase), CMK (4-(cytidine 5’diphospho)-2-C-methyl-D-erythritol kinase), MDS (2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase), HDS (4-hydroxy-3-methylbut-2-enyl-diphosphate synthase), HDR (4-hydroxy-3-methylbut-2-enyl diphosphate reductase), and IPPI (isopentenyl diphosphate Δ-isomerase). 1, casbene; 2, 16-hydroxy-casbene; and 3 and 4, undetermined compounds.