Skip to main content
. 2021 Oct 14;24(11):103282. doi: 10.1016/j.isci.2021.103282

Figure 4.

Figure 4

DNAJB6-enriched sEVs suppress polyQ aggregation in vivo

(A) Experimental workflow for sEV administration to R6/2 mice and HTT staining of brain and spinal cord tissue. 10 μL of a 5mg/mL sEV (XP-GFP-DNAJB6 or XP-GFP) formulation (total 50 μg protein) was used per injection.

(B) Immunohistochemistry images of HTT aggregates in striatum and spinal cord tissues. Note the decrease in aggregates (brown spots) in tissues of XP-GFP-DNAJB6 sEV-treated animals. Insets in (B) show magnified view of selected cells.

(C and D) Quantification of HTT aggregates in brain and spinal cord tissues for XP-GFP and XP-GFP-DNAJB6 sEV-treated animals. HTT aggregation is significantly reduced in striatum in XP-GFP-DNAJB6 sEV-treated animals as compared with animals with XP-GFP sEV treatment. Data are represented as mean ± SD of n = 6 animals (∗p < 0.05; ns, nonsignificant; Wilcoxon test). Hematoxylin was used for nuclear staining. XP: XPack. Scale bar: 50 μm.