Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 20;12:697071. doi: 10.3389/fimmu.2021.697071

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Zhao, Jiang, Sun, Wang, Hu, Zhu, Yin, Chen, Ma, Zhao and Luan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

rhHMGB1-induced endothelial barrier hyperpermeability and RhoA/ROCK1 expression in ECs. (A) Cell viability of HPMEC was evaluated by CCK-8 measurement after stimulated with different concentration of rhHMGB1 for 24 h. (B) HPMECs were stimulated with the indicated concentrations of rhHMGB1 for 24 h. (C) HPMECs were stimulated with 600 ng/ml rhHMGB1 for the indicated times. (D, E) Immunofluorescence location of F-actin, VE-cadherin and ZO-1 in HPMECs was detected after 600 ng/ml rhHMGB1 stimulation for the indicated times. The fluorescence intensity of F-actin, VE-cadherin and ZO-1 was quantitatively analyzed using the Image J software. (F) The concentration of 600 ng/ml rhHMGB1 could selectively downregulate the expression level of VE-cadherin and ZO-1 at 24 h. (G) Time course of rhHMGB1-mediated increase in RhoA activity. Western blots showed the content of GTP-bound RhoA and total RhoA in cell lysate. (H) rhHMGB1 (600 ng/ml) treatment significantly upregulated ROCK1 expression in HPMECs at 60 min. (I) Treatment with 600 ng/ml rhHMGB1 could transiently promote the expression of pMLC. Values were shown as mean ± SD of 3 independent trials. *p < 0.05 vs. control.