Figure 4.

Broad antibody response at week 34. (A) Overview of broad HIV-Envelope specific IgG response at week 34. (B) Heterologous neutralizing antibodies elicited by VC10014 DNA plasmid and protein vaccine regimen. Rhesus plasma samples at week 34 were tested for neutralization in a TZM-bl assay. Neutralization data are expressed as ID₅₀, the plasma dilution that neutralized 50% of the infecting virus. (C) Heat maps showing Ab-dependent cellular phagocytosis (ADCP, left panel), Ab-dependent complement deposition (ADCD, middle panel) and Ab-dependent cellular cytotoxicity (ADCC, right two panels) between CD4d, Imm, and NI groups. (D) Competition ELISA showing the inhibition of CD4bs-specific VRC01 mAb to HIV envelope in rhesus plasma. (E) ELISA showing autologous F8 gp140 specific IgG and heterologous Bal gp120 specific IgG in buccal mucosa of CD4d, Imm, and NI rhesus macaques. Red = CD4 depleted (CD4d) group, turquoise = Immunized (Imm) group, black = Non-Immunized (NI) group. * indicates significance (p < 0.05) as determined by unpaired t-test comparing Imm and CD4d groups.