Figure 4.

Modeling and quantification of cofactor requirements and abundances of proteins in iron-sulfur (Fe/S) cluster biosynthesis

Predicted requirement of (A) biotin, ferroheme B, heme A and lipoic acid, and (B) siroheme and (C) iron-sulfur clusters at increasing growth rate. (D) Overview of mitochondrial iron-sulfur (Fe/S) cluster biosynthesis in yeast. Synthesis can be divided into three steps, the first being the synthesis of a [2Fe-2S] cluster on a scaffold protein (Isu1 or Isu2) which is essential for synthesis of all clusters. The [2Fe-2S] cluster is then transferred to glutaredoxin 5 (Grx5), followed by the insertion of the cluster into target apo-proteins or further transfer to the machinery responsible for synthesizing [4Fe-4S] clusters. (E) Comparison of predicted abundances of the Fe/S cluster biosynthetic machinery and proteomics data at a growth rate of 0.1 h⁻¹ (Lahtvee et al., 2017). (F-G) Predicted abundances of the mitochondrial Fe/S cluster biosynthetic machinery at increasing growth rates.

See also Figure S3