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. 2021 Oct 12;24(11):103262. doi: 10.1016/j.isci.2021.103262

Figure 2.

Figure 2

Doxycycline induced adipocyte-specific NaKtide expression improves oxidative stress in visceral adipose tissue of mice fed a WD

(A) NaKtide concentration by competitive ELISA.

(B–D)(B) Protein carbonylation assay. Immunoblot analysis of (C) α-1 subunit and (D) α-2 subunit with data shown as mean band density normalized to GAPDH.

(E) pSrc immunoblot analysis with data shown as mean band density normalized to c-Src.

(F–J)(F) Immunoblot analysis of pERK 1/2 with mean band density normalized to total ERK. mRNA expression of (G) TNFα, (H) MCP1, (I) Nos3 and (J) GPx1. Results are expressed as means ± SEM. N = 4–6/group. Statistical analysis by one-way ANOVA, multiple comparison using Tukey's test, where ∗p < 0.05 vs. CTR, ∗∗p < 0.01 vs. CTR, #p < 0.05 vs. CTR + NaKtide, ##p < 0.01 vs. CTR + NaKtide, &p < 0.05 vs. WD, &&p < 0.01 vs. WD.