Figure 4.

Doxycycline induced adipocyte-specific NaKtide expression improves cellular transcriptomic profile and biological pathways in the hippocampus of mice fed a WD

(A) Volcano plots of gene expression in WD vs. Control (left panel) and WD + NaKtide vs. WD (right panel) plotting antilog of unadjusted p value on y axis vs. log₂ Fold Change on x axis. Genes downregulated (unadjusted p value of <0.1) by WD colored orange and genes upregulated (unadjusted p value of <0.1) by WD colored blue. We note that doxycycline induced NaKtide expression “moved” genes dysregulated with a high fat diet in the opposite direction.

(B) Validation of differential expression of selected genes from RNAseq analysis by qRT-PCR in the hippocampus. Genes validated by qRT-PCR includes akt3, ppara, il1r, fabp4, fabp1, irs1, rasa1 and itga5. Results expressed as log₂ values of the fold change (±SEM) show similar up- or down regulation of these genes by qRT-PCR as compared to the gene expression by RNAseq in mice fed WD with or without doxycycline induced NaKtide. N = 6/group.