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. 2021 Oct 20;12:734988. doi: 10.3389/fendo.2021.734988

Figure 2.

Figure 2

Growth plate dynamics in the tibial growth plate of mouse knee joints. Growth plate zone width of non-operated and DMM, or non-loaded and loaded knee joints of C57BL/6 male mice (A). Ten measurements per section were obtained along the length of the tibial growth plate in the middle region of the knee joint (n=4 mice for each experimental group). Immunohistochemical labelling for type X collagen (Col10a1) and matrix metalloproteinase (MMP13) in the 6μm coronal sections of growth plate of DMM and non-operated (B), or mechanically loaded and non-loaded (C) middle regions of knee joints of C57BL/6 male mice. White arrows indicate examples of positive labelling, and black arrows indicate a lack of labelling, in the tibiae. Images are representative of results in 4 individual mice. Scale bar = 100μm. PZ, proliferative zone; HZ, hypertrophic zone ****p < 0.0001.