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. 2021 Oct 20;12:763647. doi: 10.3389/fimmu.2021.763647

Figure 1.

Figure 1

T cell depletion suppresses heart failure at late phase but promotes hypertrophy at early phase after TAC. (A) Changes in numbers of CD45+cells, the numbers, and the population of CD4+T and CD8+T cells gated on CD45+TCRβ+ cells isolated from heart after TAC. Day 0 represents the measurement before TAC. P values vs. day0. (n=4 in each point) (B-D) Echocardiography up to 8 weeks after TAC. 0 week indicates the measurement before TAC treatment. P values vs. WT. (WT n=7, CD3ϵ-/- n=6 After the measurement at 2w, two mice in each group were excluded for single cell analysis.) (E) Gross appearance of heart 8 weeks after TAC. (F) mRNA (Nppb and col1a1/Hprt1) levels extracted from whole heart 6 weeks after TAC. (n=6-8) (G) WGA staining 2 weeks after TAC. The cross-sectional area of the myocardium was measured by extracting the myocardium with the nucleus at its center from each slide made from n=4 in each group. Scale bar: 50µm. (H) Heart weight and heart weight/body weight 2 weeks after TAC. (n=6-8) (I) Short-axis histology of heart two and 8 weeks after TAC (Masson’s trichrome staining). Scale bar: 1mm. Data points are individual mice in one of two individual experiments, except for B-D. p values were determined by two-tailed Student’s t-test or one-way analysis of variance (ANOVA). Data are means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001.