Skip to main content
. 2021 Oct 20;12:763647. doi: 10.3389/fimmu.2021.763647

Figure 2.

Figure 2

CD8+T cell depletion results in cardioprotective hypertrophy. (A) Expression of genes related to cardiac hypertrophy and tissue repair from whole RNAseq data of cardiomyocytes 2 weeks after TAC. (n=2) (B) mRNA (Myh7/Hprt1) extracted from whole heart 2 weeks after TAC determined by q-PCR. (n=6-9) (C) Heart weight/body weight of heart 2 weeks after TAC. (n=4-9) (D) mRNA (Myh7/Hprt1) extracted from whole heart 2 weeks after TAC determined by q-PCR. (n=6-9) (E) mRNA (Nppb/Hprt1) extracted from whole heart 6 weeks after TAC determined by q-PCR. (n=4-8) For CD4+ or CD8+T cell depletion, mice received an i.p. injection of anti-CD4 antibody or anti-CD8 antibody or vehicle (PBS) on day 0 and every three days until 2 weeks after TAC. (F) WT or OT-I-CD8+T cells were transferred (TF) into CD3ϵ-/- mice five days prior to the TAC operation, then cell the number of CD8+T cells was counted in the heart 1 week after TAC. (n=4-8) (G) Heart weight/body weight 2 weeks after TAC in WT or OT-I-CD8+T cells-transferred (TF) mice. (n=7-10) (H) Short-axis histology of heart 2 weeks after TAC (H&E staining). Representative picture of samples. Scale bar: 1mm. Data points are individual mice in one of two or three individual experiments, except for RNAseq analysis. p values were determined by two-tailed Student’s t-test or one-way analysis of variance (ANOVA). Data are means ± SD. *p < 0.05, **p < 0.01.