Figure 4.

CD8⁺T cell depletion modulates macrophage status. (A) Numbers of CD11b⁺ cells, F4/80⁺ macrophages (CD11b⁺F4/80⁺), and Ly6c^low macrophages (CD11b⁺F4/80⁺Ly6c^low) gated on CD45⁺ FVD^- CD3ϵ^- CD19^- Ly6g^- cells isolated from heart 1 week after sham/TAC operation. (n=6-10) For CD4⁺ or CD8⁺T cell depletion, mice received an i.p. injection of anti-CD4 antibody or anti-CD8 antibody on day 0 and every three days until 1 week after sham/TAC. (B) Numbers of CD4⁺T and CD8⁺T cells gated on CD45⁺TCRβ⁺ cells isolated from heart after sham/TAC. (n=4 per group) (C) Numbers of CD11b⁺ cells, F4/80⁺ macrophages, and Ly6c^low macrophages isolated from spleen 1 week after TAC operation. (n=3-4) (D) scRNAseq FltSNE display of cardiac CD45-positive cells 1 week after sham/TAC operation. Classical macrophage/monocyte fractions corresponding to clusters are shown. (E) Cell distribution under each condition (cell numbers are aligned). (F) Gene expressions on the FltSNE plot. (G) Cluster compositions of each group. After calculating the % of total cells of each cluster in each sample, the cell compositions of each cluster were z-scaled, clustered based on Pearson’s correlation coefficient using hclust (according to the Ward method) and visualized using pheatmap. Data points are individual mice in one of two or three individual experiments, except for single cell analysis. p values were determined by two-tailed Student’s t-test or one-way analysis of variance (ANOVA). Data are means ± SD. **p < 0.01, ***p < 0.001 and ****p < 0.0001.