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. 2021 Oct 20;12:763647. doi: 10.3389/fimmu.2021.763647

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Copyright © 2021 Komai, Ito, Nomura, Shichino, Katoh, Yamada, Ko, Iizuka-Koga, Nakatsukasa and Yoshimura

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cardiac macrophage differentiation defined by RNA velocity analysis. (A) scRNAseq FltSNE display of cardiac monocyte/macrophage subpopulation 1 week after sham/TAC operation. (B) Indicated gene expressions on the FltSNE plot. (C) RNA velocity analysis of cardiac monocyte/macrophage clusters sham and 1 week after operation in WT, CD3ϵ^-/-, and CD8⁺T cell depleted TAC mice. Analysis was against monocyte/macrophage clusters (CD45⁺ cell clusters 1 to 7, 9, 10, 12, and 17 in Figure 4C ).