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. 2021 Oct 20;12:742370. doi: 10.3389/fimmu.2021.742370

Figure 1.

Figure 1

Mtb infection downregulates FAK expression. (A) THP-1 macrophages were infected with Mtb at a multiplicity of infection (MOI) of 10. Cell lysates were prepared at the indicated time points post-infection, and total FAK protein levels were analyzed by western blot. (B) PTK2 expression levels in THP-1 macrophages infected with Mtb as in (A) were quantified using qRT-PCR at the indicated days post infection. (C) RAW 264.7 cells were infected Mtb as in (A), and cell lysates were prepared at the indicated time post infection. Blots shown in (A, B) are representative of three independent experiments. Densitometry analysis of the blots was performed using Image Quant LAS 4000 software with intensities normalized to GAPDH. Relative fold change of total FAK levels was expressed relative to uninfected cells. (D) Ptk2 expression levels in RAW 264.7 macrophages were measured as in (B). qRT-PCR data were analysed using the ΔΔCT method normalizing to (B) ACTB or (D) GAPDH as a reference gene, and fold change is expressed relative to uninfected cells. qRT-PCR data represent the mean of three replicates. ***p < 0.001.