Skip to main content
. 2021 Oct 20;12:742370. doi: 10.3389/fimmu.2021.742370

Figure 4.

Figure 4

FAK is required to block cell death of Mtb-infected macrophages. (A) THP-1, THP-FAKi and THP-FAK+ macrophages were infected with Mtb at an MOI of 10, after which changes in adherence (viability) were monitored by RTCA as indicated by the Cell Index (CI). CI was measured continuously every hour for 120 hours post-infection. The data represent the mean of three independent biological replicates. (B) THP-1, THP-FAKi, THP-FAK+ macrophages, (C) mBMDMs, and (D) RAW 264.7 cells were infected with Mtb at an MOI of 10 and cell viability was quantified using Cell Titer-Glo (measured as Relative Luminescence Units, RLU). Data in (C, D) represent 4 days post-infection. (E) THP-1, THP-FAKi, and THP-FAK+ macrophages were infected with Mtb at an MOI of 10, and at the indicated time points post-infection, cells were stained with Fixable Viability Stain (FVS780) dye and analyzed using flow cytometry to measure the percentage of viable cells. Error bars represent the mean ± SD of three independent biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001.