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. 2021 Oct 20;12:742370. doi: 10.3389/fimmu.2021.742370

Figure 6.

Figure 6

Expression of FAK controls Mtb survival. (A) Mtb was treated with the indicated concentrations of FAK inhibitors (in the absence of macrophages), PF-573-228 and PF-562-271, for 5 days, after which Mtb growth was assessed using the resazurin viability assay. Relative viability was normalized to 100% using untreated controls. (B) THP-1, THP-FAKi, and THP-FAK+ macrophages, or (C) RAW 264.7 and RAW 264.7-FAKi macrophages were infected with Mtb-luciferase at an MOI of 10 for 6 days. Infected macrophages were lysed at the indicated time points post-infection and the resultant luminescence signal (RLU) was measured as a proxy for the relative number of viable of Mtb. (D, E) THP-1 macrophages were mock treated or pre-treated with (D) 30 µM necrostatin-1 or (E) 5 µM GSK-872 for 24 h, and subsequently infected as in (B). Infected macrophages were lysed at the indicated time points post-infection, and the resultant luminescence signal (RLU) was measured as a proxy for the relative number of viable of Mtb. Error bars represent the mean ± SD of three independent biological replicates. **p < 0.01, ***p < 0.001.