Figure 3.

Exclusion of CD5^hi and TCRαβ^hi cells from analysis enhances purity of sorted DEs

In this experiment, a PBMC sample from T1D patients was stained using our four-antibody cocktail, acquired and two gating strategies used to identify and sort DEs as shown below. Purity was determined by rerunning sorted DEs. Top panel. The plots show how inclusion of all CD5⁺ cells (left plot) and TCRαβ⁺ cells (middle plot) significantly lowers purity of sorted DEs (right plot). The number in the right plot shows percentage of bona fide DEs (19%) among sorted cells. Bottom panel. The plots show how restricting gating to CD5^lowCD19+ (left plot) and TCRαβ^low cells (middle plot) cells significantly increases purity of sorted DEs (right plot). The number in the right plot shows percentage of bona fide DEs (82%) among sorted cells.