Table 1.
Antibody cocktail used for detecting DEs
| Surface marker | Fluorophore | aSuggested dilution factor |
|---|---|---|
| CD5 | APC | 1:20 |
| CD19 | Brilliant Violent 421 | 1:20 |
| TCRαβ | Alexa Fluor 488 | 1:20 |
| IgD | PE | 1:20 |
We suggest you determine optimal concentrations of your antibodies by doing your own titrations.