REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
APC anti-human CD5 (L17F12)	BioLegend	Cat#364016; RRID: AB_2565726
BV421 anti-human CD19 (HIB19)	BD Biosciences	Cat#562440; RRID: AB_11153299
AF-488 anti-human TCRαβ (IP26)	BioLegend	Cat#306712; RRID: AB_528967
PE anti-human IgD (IA6-2)	BioLegend	Cat#348204; RRID: AB_10553900
Bacterial and virus strains
Epstein-Barr Virus B95-8	American Type Cell Culture (ATCC)	Cat#ATCC VR-1492
Biological samples
Human peripheral blood samples (8- to 80-year-old males and females)	Patients with autoimmune disease or healthy controls	N/A
Chemicals, peptides, and recombinant proteins
2-mercaptoethanol	Thermo Fisher Scientific	Cat#21985023
Antibiotic Antimycotic Solution (AAS)	Sigma Aldrich	Cat#A5955-100ML
Benzonase nuclease	Sigma Aldrich	Cat#E8263
Bovine serum albumin (BSA)	Sigma Aldrich	Cat##A1933-100G
CpG ODN 2006 (ODN 7909)	InvivoGen	Cat#tlrl-2006-1
Dimethyl Sulfoxide (DMSO)	Fisher Scientific	Cat#BP231-100
Fetal Bovine Serum (FBS)	Sigma Aldrich	Cat#F2442-500ML
Ficoll Paque Plus	MilliporeSigma	Cat#GE17-1440-02
Hanks’ Balanced Salt Solution (HBSS)	MilliporeSigma	Cat#55037C
HEPES	Thermo Fisher Scientific	Cat#15630-080
Live/Dead Fixable Aqua	Thermo Fisher Scientific	Cat#L34957
Non-essential amino acids (NEAA)	Quality Biological	Cat#116-078-721
Phosphate Buffered Saline (PBS)	Corning	Cat#46-013-CM
RPMI with L-glutamine	Corning	Cat#10-040-CV
Sodium Azide	Sigma Aldrich	Cat# S2002
Sodium Pyruvate	Thermo Fisher Scientific	Cat#11360070
Propidium Iodide	BD Biosciences	Cat#556463
Experimental models: Cell lines
IRR-MRC-5 irradiated Fibroblast	American Type Cell Culture (ATCC)	Cat#ATCC 55-X
Software and algorithms
FlowJo	TreeStar	https://www.flowjo.com/solutions/flowjo
Others
0.22 μm vacuum filtration flask	Corning	Cat#431097
70 μm cell strainers	Falcon	Cat#352350
FACS tubes	MTC Bio	Cat#T9014
FACS tubes with 35 μm cell strainer caps	Falcon	Cat#352235
Multi-color Flow cytometer	Of your choice	N/A
MoFlo Legacy High Speed Cell Sorter	Beckman Coulter (or a sorter of your choice)	beckmancoulter.com