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Hepatocyte collection and density-gradient separation
(A) Filtered hepatocytes collected from liver are centrifuged. The cartoon shows the pellet with total cells from liver.
(B) The pellet in (A) is resuspended in 50:50 Hepatocyte Attachment Medium: Gradient Solution and centrifuged. The cartoon shows the pellet containing viable hepatocytes; the supernatant is composed by dead cells and debris.
