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Hepatocyte clean up
The volume of Hepatocyte Attachment Medium for resuspending hepatocytes depends on the size of the pellet obtained in step 15.
(A) A successful isolation requires a resuspension volume of 25 mL of Hepatocyte Attachment Medium.
(B) If the yield of isolation is lower, volume can be decreased to 10 mL.
(C) If the isolation was limited, the volume of resuspension should not exceed 5 mL.
