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Hepatocyte plating
Liver cells should be plated at a confluence of 300,000 hepatocytes per 3.5 cm well for signaling experiments.
(A) Within 6 h after hepatocyte plating, cells attach to the plate and acquire a round shape (Scale bar 300 μm).
(B) 16–20 h after hepatocyte plating, cells have acquired their hexagonal shape (Scale bar 300 μm).
