Amino acid | Amount | Final concentration (stock) | Final concentration (when added to amino acid starvation medium) |
---|---|---|---|
C (L-Cystine-2HCl) | 325 mg | 3,250 mg/L | 207.52 μM |
F (L-Phenylalanine) | 75 mg | 750 mg/L | 90.81 μM |
H (L-Histidine) | 75 mg | 750 mg/L | 71.56 μM |
I (L-Isoleucine) | 250 mg | 2,500 mg/L | 381.19 μM |
K (L-Lysine-HCl) | 200 mg | 2,000 mg/L | 218.99 μM |
L (L-Leucine) | 250 mg | 2,500 mg/L | 381.19 μM |
M (L-Methionine) | 75 mg | 750 mg/L | 100.53 μM |
Q (L-Glutamine) | 1,500 mg | 15,000 mg/L | 2052.83 μM |
R (L-Arginine) | 1,000 mg | 10,000 mg/L | 1148.11 μM |
T (L-Threonine) | 100 mg | 1,000 mg/L | 167.89 μM |
V (L-Valine) | 100 mg | 1,000 mg/L | 170.72 μM |
W (L-Tryptophan) | 25 mg | 250 mg/L | 24.48 μM |
Y (L-Tyrosine-2Na-2H2O) | 145 mg | 1,450 mg/L | 160.05 μM |
ddH2O | 100 mL | N/A | N/A |
Total | 100 mL | 50× | 1× |