Amino acid	Amount	Final concentration (stock)	Final concentration (when added to amino acid starvation medium)
D (L-Aspartic Acid)	200 mg	2,000 mg/L	150.26 μM
E (L-Glutamic Acid)	200 mg	2,000 mg/L	135.93 μM
G (Glycine)	100 mg	1,000 mg/L	133.21 μM
N (L-Asparagine)	500 mg	5,000 mg/L	378.44 μM
P (L-Proline)	200 mg	2,000 mg/L	173.72 μM
S (L-Serine)	300 mg	3,000 mg/L	285.47 μM
ddH2O	100 mL	N/A	N/A
Total	100 mL	100×	1×