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. 2021 Oct 26;2021:5499963. doi: 10.1155/2021/5499963

Figure 2.

Figure 2

Overexpression of PAX8-AS1 impedes the cell proliferation and enhances apoptosis. Note. (a) The transfection efficiency of PAX8-AS1 in PTC K1 and IHH4 cells was detected by qRT-PCR; (b) MTT assay was conducted to separately detect the optical density values at 490 nm at 24, 48, and 72 h in K1 and IHH4 cells after overexpressing PAX8-AS1; (c) cell proliferation was examined in K1 and IHH4 cells after PAX8-AS1 overexpression by EdU assay; (d) flow cytometry was used to analyze the scatter patterns of cell cycle distribution of K1 and IHH4 cells after PAX8-AS1 overexpression; (e) the protein bands of apoptosis-related proteins in K1 and IHH4 cells were detected by western blot. ∗P < 0.05,  ∗∗P < 0.01, and ∗∗∗P < 0.001.