Table 1.
List of primer pairs and probe sets for target virus detection
| Set | Virus | Target | Name | Sequence (5´–3´) | Tm (℃) |
|---|---|---|---|---|---|
| 1 | Influenza A | M | Forward | ACAAGACCAATCCTGTCACCT | 63 |
| Reverse | TGGACAAAGCGTCTACGCT | 64 | |||
| Probe | FAM-CTCACCGTGCCCAGTGAGC-BHQ1 | 67 | |||
| Influenza B | NS & NEP | Forward | GGATCCTCAACTCACTCTTCGA | 63 | |
| Reverse | CGGTGCTCTTGACCAAATTGG | 64 | |||
| Probe | CAL Fluor Red 610-CAATTCGAGCAGCTGAAACTGCG-BHQ2 | 66 | |||
| 2 | Influenza A (H3N2) | HA | Forward | CCATGCAGTACCAAACGGAAC | 64 |
| Reverse | CACACTGAGGGTCTCCCAATAG | 64 | |||
| Probe | FAM-TGACCGAATTGAAGTTACTAATGCTACTGA-BHQ1 | 66 | |||
| Influenza A (pandemic 09, H1N1) | HA | Forward | GGGGTAGCCCCATTGCA | 64 | |
| Reverse | AGTGATTCACACTCTGGATTTCCC | 64 | |||
| Probe | CAL Fluor Red 610-TGGGTAAATGYAACATTGCTGGCTG-BHQ2 | 66–68 | |||
| 3 | PIV1 | HN | Forward | CAGGAAACCCAGACTGCAAC | 63 |
| Reverse | GCGTACAGTGTGGTTGTAGC | 63 | |||
| Probe | FAM-TGTCCGAGAGAATGCATATCAGGTGT-BHQ1 | 67 | |||
| PIV3 | HN | Forward | AGATGTATATCAACTGTGTTCAACTCC | 63 | |
| Reverse | GCCTTTGTAGTATATCCCTGGTCC | 64 | |||
| Probe | CAL Fluor Red 610-TGAAAGATCAGATTATGCATCATCAGGC-BHQ2 | 65 | |||
| 4 | PIV2 | HN | Forward | CAGGACTATGAAAACCATTTACCTAAGT | 63 |
| Reverse | GAAAGCAAGTCTCAGTTCAGCTAG | 63 | |||
| Probe | FAM-AATCAATCGCAAAAGCTGTTCAGTCA-BHQ1 | 66 | |||
| PIV4 | P | Forward | CCTGGAGTCCCATCAAAAGTAAG | 63 | |
| Reverse | GCATCTATACGAACACCTGCTC | 63 | |||
| Probe | CAL Fluor Red 610-TGTCTCAAAATTTGTTGATCAAGACAATACAAT-BHQ2 | 66 | |||
| 5 | hMPV | F | Forward | GTTGTGVGGCARTTTTCAGA | 60–64 |
| Reverse | CCTGCWGATGTYGGCATGT | 63–65 | |||
| Probe1 | FAM-AAYGCTGGAATAACACCAGCAAT-BHQ1 | 64–66 | |||
| Probe2 | FAM-AATGCAGGGATAACACCRGCAAT-BHQ1 | 66–68 | |||
| ADV | H | Forward | CCCATGGAYGAGYCCAC | 60–65 | |
| Reverse | TCRATGACGCCGCGGT | 65–67 | |||
| Probe1 | FAM- TTCGACGTSGTCMGAGTGCAC-BHQ1 | 67–69 | |||
| Probe2 | FAM- TTTGACGTGGTCCGTGTGCAC-BHQ1 | 67 | |||
| 6 | HRV | Poly | Forward | TGAGTCCTCCGGCCC | 62.2 |
| Reverse | GAAACACGGACACCCAAAGT | 62.8 | |||
| Probe1 | FAM-CTGAATGCGGCTAATCYWACCCC-BHQ1 | 65–68 | |||
| Probe2 | FAM-CTGAATGYGGCTAACCTTAACCCYG-BHQ1 | 65–69 | |||
| RSV | M | Forward | GGGCAAATATGGAAACATACGTGAA | 64 | |
| Reverse | GGCACCCATATTGTWAGTGATGC | 64 | |||
| Probe | CAL Fluor Red 610-CTTCACGAGGGCTCCACATACAC-BHQ2 | 66 | |||
| 7 | SARS-CoV-2 (RdRp) | RdRp | Forward | GATGATACTCTCTGACGATGCTGT | 64 |
| Reverse | TCTCAGTCCAACATTTTGCTTCAG | 64 | |||
| Probe | FAM-ATGCATCTCAAGGTCTAGTGGCTAG-BHQ1 | 65 | |||
| SARS-CoV-2 (N) | N | Forward | GCTTCAGCGTTCTTCGGAATG | 64 | |
| Reverse | RTCATCCAATTTGATGGCACCT | 63 | |||
| Probe | CAL FLUOR RED 610-TTGGCATGGAAGTCACACCTTCG-BHQ2 | 67 |