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. 2021 Oct 20;12:691867. doi: 10.3389/fphys.2021.691867

FIGURE 1.

FIGURE 1

Effect of high glucose (HG) culture medium on store-operated Ca2+ entry (SOCE) in MUS-M1 cells. (A) SOCE was evoked by a classic “Ca2+ added back” protocol. Images showing fluorescence intensity indicating the change of transient intracellular Ca2+ concentration ([Ca2+]i). Scale bars, 10 μm. Representative traces (B,D) and summarized data (C,E) showing the change in [Ca2+]i (SOCE) evoked by extracellular application of 2 mM Ca2+ after the treatment of 2 μM thapsigargin (TG) (B,C) or 100 μM carbachol (D,E) for 20 min in Ca2+-free solution to deplete internal Ca2+ stores in MUS-M1 cells cultured in normal glucose (NG, 5.6 mM D-glucose + 20 mM α-mannitol) or HG (25 mM D-glucose) medium for 1, 3, and 7 days. Values are shown as the mean ± SEM (n = 5–6); *P < 0.05, NG vs. HG on the same day.