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. 2021 Oct 20;12:691867. doi: 10.3389/fphys.2021.691867

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Copyright © 2021 Dai, Guo, Wang, Jiang, Chen, Hu, Du, Xia, Zhang and Shen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of high glucose (HG) culture medium on Orai1 and STIM1 protein expression in MUS-M1 cells. Representative immunoblotting images (A) and summarized data (B,C) showing the expression levels of Orai1 (B) and STIM1 (C) proteins in MUS-M1 cells cultured in normal glucose (NG, 5.6 mM D-glucose + 20 mM α-mannitol) or HG (25 mM D-glucose) medium for 1, 3, and 7 days. Alpha-actin was used as a loading control. Values are shown as the mean ± SEM (n = 3); **, and *** denote P < 0.01, and P < 0.001, respectively. NS means non-significant, NG vs. HG on the same day.