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. 2021 Oct 20;12:691867. doi: 10.3389/fphys.2021.691867

FIGURE 3.

FIGURE 3

Effect of Orai1 and STIM1 siRNAs on store-operated Ca2+ entry (SOCE) in MUS-M1 cells. (A,B) Representative immunoblotting images showing Orai1 and STIM1 expression levels in MUS-M1 cells transfected with Orai1 siRNA (A), STIM1 siRNA (B), or scrambled control siRNA. **, and *** denote P < 0.01, and P < 0.001, respectively. Representative traces (C,E) and summarized data (D,F) showing the alterations in [Ca2+]i (SOCE) evoked by extracellular application of 2 mM Ca2+ after the treatment of 100 μM carbachol for 10 min in Ca2+-free solution to deplete internal Ca2+ stores in MUS-M1 cells cultured in normal glucose (NG, C,D, 5.6 mM D-glucose + 20 mM α-mannitol) or high glucose (HG, E,F, 25 mM D-glucose) medium for 7 days. Alpha-actin was used as a loading control. Values are shown as the mean ± SEM (n = 5–6); *P < 0.05, scrambled siRNA vs. Orai1 or STIM1 siRNA transfections.