Table 4. Composition of the basal diet (ingredients) and analyzed nutrient contents given to broilers chickens (25–42 days).
| Ingredient (%) | Dietary treatment | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | |
| Com grain | 62.66 | 62.66 | 60.51 | 60.51 | 57.92 | 57.92 | 65.41 | 65.41 | 63.00 | 63.00 | 60.27 | 60.27 | 59.92 | 59.92 | 57.76 | 57.76 | 55.64 | 55.65 |
| Soybean meal (44% crude protein) | 27.77 | 27.77 | 27.70 | 27.70 | 23.95 | 23.95 | 27.21 | 27.21 | 25.00 | 25.00 | 20.00 | 20.00 | 28.33 | 28.33 | 28.26 | 28.26 | 27.91 | 27.86 |
| Pomegranate peel | 0.00 | 0.00 | 4.00 | 4.00 | 8.00 | 8.00 | 0.00 | 0.00 | 4.00 | 4.00 | 8.00 | 8.00 | 0.00 | 0.00 | 4.00 | 4.00 | 8.00 | 8.00 |
| Waste oil | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 4.00 | 4.00 | 4.00 | 4.00 | 4.00 | 4.00 |
| Vitamin E | 0.00 | 0.02 | 0.00 | 0.02 | 0.00 | 0.02 | 0.00 | 0.02 | 0.00 | 0.02 | 0.00 | 0.02 | 0.00 | 0.02 | 0.00 | 0.02 | 0.00 | 0.02 |
| Soybean oil | 3.00 | 3.00 | 3.00 | 3.00 | 3.00 | 3.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| Gluten meal | 0.00 | 0.00 | 0.00 | 0.00 | 2.48 | 2.48 | 0.00 | 0.00 | 1.44 | 1.44 | 4.76 | 4.74 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| Dicalcium phosphate | 1.46 | 1.46 | 1.45 | 1.45 | 1.47 | 1.47 | 1.46 | 1.46 | 1.47 | 1.47 | 1.50 | 1.50 | 1.47 | 1.47 | 1.45 | 1.45 | 1.45 | 1.45 |
| Oyster sell | 1.01 | 1.01 | 0.99 | 0.99 | 0.97 | 0.97 | 1.02 | 1.02 | 1.00 | 1.00 | 0.98 | 0.98 | 1.01 | 1.01 | 0.98 | 0.98 | 0.96 | 0.96 |
| Bicarbonate Na | 0.32 | 0.32 | 0.31 | 0.31 | 0.35 | 0.35 | 0.33 | 0.33 | 0.35 | 0.35 | 0.41 | 0.40 | 0.32 | 0.32 | 0.31 | 0.31 | 0.34 | 0.35 |
| DL-methionine1) | 0.33 | 0.33 | 0.33 | 0.33 | 0.32 | 0.32 | 0.32 | 0.32 | 0.32 | 0.32 | 0.30 | 0.30 | 0.33 | 0.33 | 0.33 | 0.33 | 0.35 | 0.35 |
| L-Lysine HCl2) | 0.32 | 0.32 | 0.32 | 0.32 | 0.42 | 0.42 | 0.33 | 0.33 | 0.39 | 0.39 | 0.53 | 0.53 | 0.31 | 0.31 | 0.31 | 0.31 | 0.40 | 0.42 |
| L-Threonine3) | 0.15 | 0.15 | 0.16 | 0.16 | 0.18 | 0.18 | 0.15 | 0.15 | 0.17 | 0.17 | 0.20 | 0.20 | 0.15 | 0.15 | 0.15 | 0.15 | 0.17 | 0.17 |
| Vitamin premix4) | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 |
| Mineral premix5) | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 |
| Common salt | 0.25 | 0.25 | 0.25 | 0.25 | 0.22 | 0.22 | 0.24 | 0.24 | 0.22 | 0.22 | 0.18 | 0.18 | 0.25 | 0.25 | 0.25 | 0.25 | 0.23 | 0.22 |
| K2SO4 | 0.00 | 0.00 | 0.00 | 0.00 | 0.14 | 0.14 | 0.00 | 0.00 | 0.07 | 0.07 | 0.30 | 0.30 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| Salinomycin | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 |
| Inert6) | 2.18 | 2.16 | 0.43 | 0.41 | 0.03 | 0.01 | 0.98 | 0.96 | 0.02 | 0.00 | 0.02 | 0.03 | 3.36 | 3.34 | 1.65 | 1.63 | 0.00 | 0.00 |
| Calculated chemical composition | ||||||||||||||||||
| Apparent metabolizable energy (kcal/kg) | 2,990 | 2,990 | 2,990 | 2,990 | 2,990 | 2,990 | 2,990 | 2,990 | 2,990 | 2,990 | 2,990 | 2,990 | 2,990 | 2,990 | 2,990 | 2,990 | 2,990 | 2,990 |
| Calcium (%) | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 |
| Available phosphorus (%) | 0.39 | 0.39 | 0.39 | 0.39 | 0.39 | 0.39 | 0.39 | 0.39 | 0.39 | 0.39 | 0.39 | 0.39 | 0.39 | 0.39 | 0.39 | 0.39 | 0.39 | 0.39 |
| Sodium (%) | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 |
| Chlorine (%) | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 |
| Potassium (%) | 0.74 | 0.74 | 0.74 | 0.74 | 0.74 | 0.74 | 0.74 | 0.74 | 0.73 | 0.73 | 0.73 | 0.73 | 0.74 | 0.74 | 0.74 | 0.74 | 0.73 | 0.73 |
| Linoleic acid (%) | 2.92 | 2.92 | 2.88 | 2.88 | 2.81 | 2.81 | 2.50 | 2.50 | 2.44 | 2.44 | 2.36 | 2.35 | 3.35 | 3.35 | 3.30 | 3.30 | 3.25 | 3.25 |
| Lysine (%) | 1.15 | 1.15 | 1.15 | 1.15 | 1.15 | 1.15 | 1.15 | 1.15 | 1.15 | 1.15 | 1.15 | 1.15 | 1.15 | 1.15 | 1.15 | 1.15 | 1.21 | 1.22 |
| Methionine + cysteine (%) | 0.90 | 0.90 | 0.90 | 0.90 | 0.90 | 0.90 | 0.90 | 0.90 | 0.90 | 0.90 | 0.90 | 0.90 | 0.90 | 0.90 | 0.90 | 0.90 | 0.91 | 0.91 |
| Threonine (%) | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 |
| Tryptophan (%) | 0.24 | 0.24 | 0.24 | 0.24 | 0.22 | 0.22 | 0.24 | 0.24 | 0.22 | 0.22 | 0.20 | 0.20 | 0.24 | 0.24 | 0.24 | 0.24 | 0.23 | 0.23 |
| Analyzed composition | ||||||||||||||||||
| Crude protein (%) | 18.20 | 18.24 | 18.19 | 18.21 | 18.18 | 18.20 | 18.24 | 18.22 | 18.21 | 18.18 | 18.16 | 18.22 | 18.25 | 18.23 | 18.20 | 18.19 | 18.26 | 18.21 |
| Ether extract (%) | 2.59 | 2.62 | 2.61 | 2.52 | 2.56 | 2.59 | 2.65 | 2.69 | 2.68 | 2.58 | 2.63 | 2.70 | 2.49 | 2.45 | 2.37 | 2.47 | 2.45 | 2.41 |
| Crude Fiber (%) | 3.32 | 3.36 | 3.98 | 3.92 | 4.45 | 4.41 | 3.31 | 3.37 | 3.91 | 3.87 | 4.25 | 4.19 | 3.29 | 3.34 | 4.00 | 3.95 | 4.58 | 4.63 |
DL-Methionine, Feed Grade 99% (Evonik methionine, Essen, Germany).
L-Lysine Feed Grade 98.5% (Alborz Gostar Darou, Karaj, Iran).
L-Threonine, Feed Grade 98.5% (Evonik methionine, Essen, Germany).
Vitamin mixture provided per kilogram of diet: 15,000 IU vitamin A (retinol), 3,750 IU vitamin D3 (cholecalciferol), 20.20 mg vitamin E (alpha-tocopherol), 3.7 mg vitamin K3 (menadione), 3 mg vitamin B1 (thiamine), 7.5 mg of vitamin B2 (riboflavin), 55 mg vitamin B3 (niacin), 4.5 mg of vitamin B6 (pyridoxine), 1 mg vitamin B7 (biotin), 15 mg vitamin B12 (cobalamin), and 11.5 mg of vitamin B5 (pantothenic acid).
Mineral mixture provided per kilogram of diet: 50 mg Mn (manganese), 47 mg Zn (zinc oxide), 25 mg Fe (iron), 7.5 mg Cu (copper sulfate), 2.6 mg I (iodine), and 0.40 mg Se (selenium).
Grit stone was used in the rations.