Table 2. Computer-assisted semen analysis (CASA) program setting.

Parameter	Setting
Field-of-view depth=Depth of sample chamber	20 µm
Light adjustment	90–105
Total number of cells evaluated or number of fields	1,500 spermatozoa or 14 fields
Sperm recognition area	15–75 µm2
Frame rate	25 frames/sec
Points assessed for sperm motility	11, VAP points 5
Total motility	Progressive motility + local motility
Local motility	DSL (distance: straight) < 6.0 µm
Progressive motility	> 75% of STR (straightness)