Fig. 1. Effects of Met supplementation on the mRNA levels of apoptosis-related genes in MAC-T cells under hyperthermia (42°C, 6 h).
The mean values of mRNA levels in CON were set to 1.00. Data with superscript letters are significantly different from each other (p < 0.05). Data are mean ± SEM, n = 6. CON, normal amino acid profile (60 μg/mL of Met in medium); MET70, normal amino acid profile plus 10 μg/mL Met (70 μg/mL of Met in medium); MET8, normal amino acid profile plus 20 μg/mL Met (80 μg/mL of Met in medium); MET90, normal amino acid profile plus 30 μg/mL Met (90 μg/mL of Met in medium). Met, methionine; HSP70, heat shock protein 70; BAX, Bcl-2-associated X protein; BCL2, B-cell lymphoma-2; MAC-T, mammary alveolar cells-large T antigen.
