Skip to main content
. 2021 Sep 30;63(5):1126–1141. doi: 10.5187/jast.2021.e93

Fig. 3. Effects of Met supplementation on the levels of mTOR pathway-related proteins in MAC-T cells under hyperthermia (42°C, 6 h).

Fig. 3.

(A) Relative levels of total proteins; (B) relative levels of phosphorylated proteins; (C) ratios of phosphorylated proteins to total proteins. The mean values of total and phosphorylated protein levels in the CON were set to 1.00. Data with superscript letters are significantly different from each other (p < 0.05). Data are mean ± SEM, n = 6. CON, normal amino acid profile (60 μg/mL of Met in medium); MET70, normal amino acid profile plus 10 μg/mL Met (70 μg/mL of Met in medium); MET80, normal amino acid profile plus 20 μg/mL Met (80 μg/mL of Met in medium); MET90, normal amino acid profile plus 30 μg/mL Met (90 μg/mL of Met in medi-um). Met, methionine; t-AKT, total AKT; t-mTOR, total mechanistic target of rapamycin kinase; t-RPS6KB1, total ribosomal protein S6 kinase B1; t-RPS6, total ribosomal protein S6; p-AKT, phos-phorylated AKT; p-mTOR, phosphorylated mechanistic target of rapamycin kinase; p-RPS6KB1, phosphorylated ribosomal protein S6 kinase B1; p-RPS6, phosphorylated ribosomal protein S6; mTOR, mechanistic target of rapamycin; MAC-T, mammary alveolar cells-large T antigen.