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. 2021 Sep 30;63(5):984–997. doi: 10.5187/jast.2021.e90

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© Copyright 2021 Korean Society of Animal Science and Technology

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — (A) DNA damage was evaluated by detecting DNA double strands breaks using an antibody against H2A.X (green), and DNA was stained with DAPI (red). (B) Quantitative analysis of levels of DNA damage (H2A.X fluorescence intensity) in the nuclei of oocytes (including polar bodies). (C) DNA damage repair was detected using an antibody against RAD51 (red) and DNA was stained with DAPI (blue). (D) Quantitative analysis of DNA damage repair (RAD51 fluorescence intensity) in the nuclei of oocytes (including polar bodies). The numbers of embryos tested in each group are shown as bars. ^a–cDifferent letters above the bars indicate statistically significant differences (p < 0.05). Scale bars represent 50 μm in A and C. IVA, in vitro aging; DAPI, 4’,6-diamidino-2-phenylindole.