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. 2021 Sep 30;63(5):984–997. doi: 10.5187/jast.2021.e90

Fig. 5. Developmental capacity of porcine aged oocytes.

Fig. 5.

(A) Images of embryos derived from the control, IVA 24 h and IVA 48 h groups. (B) Developmental potential of porcine embryos. Because IVA 48 h group failed to develop to blastocyst stage, the analyses presented in panel C, D and E were not performed for the IVA 48 h group. (C) Diameter of blastocysts. (D) EdU staining of porcine blastocysts. The green fluorescence shows EdU-positive cells and red fluorescence shows all nuclei of blastocysts. (E) TC, EdU-positive cell number and the ratio of the EdU-positive cell number to the TC. The numbers of embryos tested in each group are shown as bars. a–dDifferent letters above the bars indicate statistically significant differences (p < 0.05). Scale bars represent 500 μm in A and 100 μm in D. IVA, in vitro aging; PA, parthenogenetic activation; TC, total cell number; EdU, 5-ethynyl-2’-deoxyuridine.