Table 1. Primer sequences for mRNA amplification.
| Gene name (Symbo) | GeneBank ID | Sequences (5’→3’) | Product size (bp) |
|---|---|---|---|
| Glyceraldehydes 3-phosphate (GAPDH)1) | KJ786424 | F :
TCGGAGTGAACGGATTTGG R : GTAGTGGAGGTCAATGAAGGG |
110 |
| Actin alpha 1, skeletal muscle (ACTA1)1) | NM_001167795 | F :
GGTCAGAAAGATTCCTACGTGG R : GCTCGTTGTAGAAGGTGTGG |
139 |
| Transforming growth factor beta 1 (TGFb1) | NM_214015 | F :
GCTACCATGCCAATTTCTGC R : GGACCTTGCTGTACTGAGTG |
72 |
| Connective tissue growth factor (CTGF) | U70060 | F :
AATCTCCAAGCCCGTCAAG R : ACACTTGAACTCCACAGGAAG |
139 |
| Periostin (POSTN) | KF500034 | F :
GATTCCTTTCTTACCCGTATTTTCTC R : GTTGAAGGGCACAGACATTTG |
140 |
| Collagen type I alpha 1 chain (COL1A1) | LC223106 | F :
ACATCAGCAAGAACCCCAAG R : GTACTCGAACTGGAATCCGTC |
77 |
| Collagen type III alpha 1 chain (COL3A1) | NM_001243297 | F :
AGAGCGGAGAATATTGGGTTG R : CTTACGTGGAACAGTAGAAGGAC |
122 |
| TIMP metallopeptidase inhibitor 1 (TIMP1) | NM_213857 | F :
CTCGTACCAGCGTTATGAGATC R : GTGTAGATGAACCGGATGTCAG |
90 |
| Activin A receptor type 2A (Acta2a) | NM_001204765 | F :
CGAATCCAGTTACACCTAAGCC R : ACACCCAAAACGCACAAATG |
102 |
| Bone morphogenetic protein 7 (BMP7) | NM_001105290 | F :
TTCCCACTCAACTCGTACATG R : GAAGTAGAGGACGGAAATGGC |
139 |
1)
Housekeeping genes.