Table 4. The survival of Lactobacillus brevis KT38-3 and some biological activity test results to evaluate its probiotic potential.
| Probiotic characteristics of L. brevis KT38-3 | ||
|---|---|---|
| Survival tests: | ||
| Survival at different pH levels (for 4 h) | pH 2 | ++ |
| pH 3 | +++ | |
| pH 4 | +++ | |
| pH 5 | +++ | |
| pH 6 | +++ | |
| pH 7 | +++ | |
| Control1) | +++ | |
| Survival at bile salts for 6 h | 0.3% | +++ |
| 0.5% | +++ | |
| 1.0% | +++ | |
| Control1) | +++ | |
| Survival (%) in simulated gastric juice (for 4 h) | pH 2 | 93.0±1.2 |
| Survival (%) in simulated gastrointestinal juice (for 6 h) | pH 7 | 78.0±1.5 |
| Cell surface hydrophobicity (%) | 85.0±4.2 | |
| Auto-aggregation (%) | 1 h | 48.3±1.3 |
| 2 h | 51.1±0.9 | |
| 4 h | 59.4±0.5 | |
| Co-aggregation (%) | 1 h | 35.1±1.3 |
| 2 h | 36.8±0.9 | |
| 4 h | 37.4±0.5 | |
| DPPH scavenging activity (%) | 71.0±2.9 | |
| ABTS scavenging activity (%) | 54.1±1.5 | |
| Virulence factors: | ||
| DNase production | np | |
| Hemolysin production | np | |
1)
The culture of L. brevis KT38-3 in MRS broth (pH=5.8) was used as a growth control for survival tests. +++, good growth; ++, slightly weak growth; +, weak growth; −, no growth; np, not produced; DPPH, 1,1-diphenyl-2-picrylhydrazyl; ABTS, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic).