Fig. 5.
Involvement of TAT in mitochondrial ROS production. (A) HeLa cells treated with or without 25 μm TAT were stained with a reduced form of MitoTracker Orange, CMH2TMRos, to detect intracellular ROS using fluorescence microscopy. (B) C. elegans carrying the gfp‐tagged hsp‐6 transgene, which encodes a protein whose expression is up‐regulated in the UPRmit, was treated with or without 50 μm TAT for 24 h, and the GFP expression levels were compared by western blotting. Tubulin represents the loading control. (C) 50 μm TAT treatment slightly, but significantly extended the average lifespan of wild‐type N2 C. elegans over that of control, untreated worms. (D) 50 μm TAT treatment significantly shortened the average lifespan of oxidative stress‐sensitive sek‐1 MAPK kinase mutant worms compared with that of controls (n = 3; *P < 0.1, **P < 0.05, Student's t‐test).