Fig. 3.

FTH1 knockdown promotes mammosphere formation efficiency and cell migration in BCa cells. (A) Mammosphere formation was evaluated in MDA‐MB‐231, JIMT1, SKBR3, and MCF‐7 cells transfected with control or FTH1‐specific siRNA and cultured in ultra‐low attachment plates for 7 days post‐transfection (20× magnification). (B) Error bars represent the mean ± SD of mammosphere numbers based on four independent experiments. (C) Wound‐healing assay was performed in MCF‐7 cells transfected with control or FTH1‐specific siRNA for 72 h post‐transfection (20× magnification); scale bar represents 200 μm. (D) Mean ± SD of wound‐healing area at 24 h postwound administration based on five independent experiments. Statistical analysis was performed using the Student t‐test; *P < 0.05; ^# no significant difference.