Fig. 5.

Silencing of c‐Myc and/or G9a upregulates FTH1 and inhibits BCa cell growth. Cell growth was assessed by (A) MTT and (B) CV staining in c‐MYC‐ and/or G9a‐silenced MDA‐MB‐231, MCF‐7, SKBR3, and JIMT1 cells at 72 h post‐transfection; error bars in A represent the mean ± SD based on four independent experiments. (C–F) Mean ± SD of CV absorbance values based on three independent experiments. t‐Test was performed. *P < 0.05; **P < 0.005. (G) Expression of FTH1, c‐MYC, and G9a in c‐MYC‐silenced MDA‐MB‐231, SKBR3, and MCF‐7 cells at 72 h post‐transfection; cells transfected with control siRNA served as negative control; β‐actin was used as a loading control.