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. 2021 Oct 20;12:757194. doi: 10.3389/fphar.2021.757194

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Copyright © 2021 Wang, Li, Mao, Mo, Cao, Luo, Zhou, Liu, Zhang and Yu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Liver tissue slice morphology and molecular mechanism of liver tumor occurrence and development. (A) H&E staining observation of morphological changes of liver tissue sections of mice in different treatment groups, (B) immunohistochemical detection of CTLA-4 and other immune checkpoint protein distribution, (C)Western blot detection of CTLA-4 and other immune checkpoint-related protein band diagram, (D) histogram, (E) RT-PCR detects the expression of mRNA related to immune checkpoints. n = 3, **p < 0.01,***p < 0.001, 1) control group, 2) SD group, 3) HCC group, 4) SD-HCC group.