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. 2021 Oct 20;12:742673. doi: 10.3389/fmicb.2021.742673

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Copyright © 2021 Zhao, Song, Wang, Zhang, Zhao and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Biological consequences of the deletion of chu_3238. (A) The wild type (WT), the Δ3238 mutant, and the C3238 cells inoculated on Stanier agar with 30 μg/ml Congo red. (B) Extraction of lipopolysaccharides (LPS) by the hot aqueous-phenol method and visualization with silver stain. Difference of LPS among WT, the Δ3238 mutant, and C3238 was marked with red brace. (C) Disk diffusion susceptibility assay for the WT and the Δ3238 mutant. DTT, dithiothreitol; Amp, ampicillin; CHP, cumene hydroperoxide. All experiments were performed in triplicate, and the error bars indicated standard errors. Significance reported as **p < 0.01, ***p < 0.001.