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. 2021 Oct 20;12:742673. doi: 10.3389/fmicb.2021.742673

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Copyright © 2021 Zhao, Song, Wang, Zhang, Zhao and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Subcellular location of CHU_3238 and co-immunoprecipitation with 3238-FLAG. (A) Western blot of outer membrane (OM) and total membrane (TM) proteins of 3238-FLAG. (B) Co-immunoprecipitation with 3238-FLAG using anti-Flag affinity gel. The arrowheads indicate the different proteins between the WT and 3238-FLAG identified by LC-MS/MS. The asterisk indicates CHU_3238 with the FLAG tag. M, protein marker, kDa. The wild type (WT) was used as the negative control. All experiments were performed in triplicate. The full-size SDS-PAGE gel and PVDF membrane are shown in Supplementary Figures 4, 5.