. 2021 Oct 20;12:742673. doi: 10.3389/fmicb.2021.742673

TABLE 1.

Strains and plasmids used in this study.

Strains and plasmids	Description^a	References or source
Strains
C. hutchinsonii strains
ATCC 33406	Wild type	ATCC33406
Δ3238	Deletion mutant of chu_3238	This study
C3238	Complementation of Δ3238 mutant	This study
3238-FLAG	Fusion of FLAG-tag at the C-terminal of chu_3238	This study
E. coli strains
DH5α	Strain used for plasmid replication	TaKaRa
Plasmids
pSJHC	Gene-targeting template plasmid carrying cat under the control of the ompA promoter from F. johnsoniae; Ap^r (Cm^r)	Wang et al., 2014
pSJHS	Gene-targeting template plasmid carrying ermF; Ap^r (Em^r)	Wang et al., 2014
pCFXSK3328	Plasmid used for deletion of chu_3328; Ap^r (Cf^r)	Guan et al., 2018
pCFXSK3328-3238	Plasmid constructed from pCFXSK3328 used for complementation of Δ3238; Ap^r (Cf^r)	This study
pSJHS-3238FLAG	Plasmid constructed from pSJHS used for complementation of chu_3238 with FLAG-tag; Ap^r (Em^r)	This study

^aAntibiotic resistance phenotypes in parentheses are expressed in C. hutchinsonii, and phenotypes not in parentheses are expressed in E. coli (Ap^r, ampicillin resistance; Cm^r, chloramphenicol resistance; Em^r, erythromycin resistance; Cf^r, cefoxitin resistance).