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. 2021 Oct 20;12:693932. doi: 10.3389/fimmu.2021.693932
A
Time point Gene Factors P value P value summary Interaction
6h lgbp EO (Melaleuca alternifolia) 0.0105 * -
challenge 0.9619 ns
Interaction 0.7353 ns
dscam EO (M. alternifolia) 0.0842 ns synergistic
challenge 0.0055 **
Interaction 0.0253 *
hsp 70 EO (M. alternifolia) 0.8680 ns synergistic
challenge 0.0104 *
Interaction 0.0434 *
hmgb EO (M. alternifolia) 0.3109 ns antagonistic
challenge 0.8372 ns
Interaction 0.0374 *
sod EO (M. alternifolia) 0.2526 ns antagonistic
challenge 0.0965 ns
Interaction 0.0155 *
pxn EO (M. alternifolia) 0.6615 ns -
challenge 0.1316 ns
Interaction 0.1670 ns
tgase1 EO (M. alternifolia) 0.2742 ns antagonistic
challenge 0.0490 *
Interaction 0.0291 *
tgase2 EO (M. alternifolia) 0.2633 ns -
challenge 0.8349 ns
Interaction 0.1402 ns
12h lgbp EO (M. alternifolia) 0.0033 ** -
challenge 0.5643 ns
Interaction 0.4659 ns
dscam EO (M. alternifolia) 0.1460 ns -
challenge 0.1796 ns
Interaction 0.8368 ns
hsp 70 EO (M. alternifolia) 0.6845 ns -
challenge 0.0494 *
Interaction 0.3857 ns
hmgb EO (M. alternifolia) 0.7357 ns -
challenge 0.0471 *
Interaction 0.9375 ns
sod EO (M. alternifolia) 0.6114 ns -
challenge 0.1680 ns
Interaction 0.9285 ns
pxn EO (M. alternifolia) 0.1248 ns -
challenge 0.0947 ns
Interaction 0.0745 ns
tgase1 EO (M. alternifolia) 0.4522 ns -
challenge 0.1306 ns
Interaction 0.1762 ns
tgase2 EO (M. alternifolia) 0.1888 ns -
challenge 0.3352 ns
Interaction 0.6450 ns
24h lgbp EO (M. alternifolia) 0.0035 ** -
challenge 0.3355 ns
Interaction 0.1960 ns
dscam EO (M. alternifolia) 0.0450 * -
challenge 0.5783 ns
Interaction 0.1452 ns
hsp 70 EO (M. alternifolia) 0.8309 ns antagonistic
challenge 0.3049 ns
Interaction 0.0324 *
hmgb EO (M. alternifolia) 0.4211 ns -
challenge 0.0818 ns
Interaction 0.9547 ns
sod EO (M. alternifolia) 0.0112 * -
challenge 0.3028 ns
Interaction 0.1879 ns
pxn EO (M. alternifolia) 0.0444 * -
challenge 0.0269 *
Interaction 0.0866 ns
tgase1 EO (M. alternifolia) 0.0002 *** -
challenge 0.0173 *
Interaction 0.1867 ns
tgase2 EO (M. alternifolia) 0.0017 ** -
challenge 0.6760 ns
Interaction 0.7809 ns
B
Time point Gene Factors P value P value summary Interaction
6h lgbp EO (Litsea citrata) 0.6656 ns -
challenge 0.9053 ns
Interaction 0.6053 ns
dscam EO (L. citrata) 0.5382 ns -
challenge 0.2184 ns
Interaction 0.9115 ns
hsp 70 EO (L. citrata) 0.5327 ns -
challenge 0.1660 ns
Interaction 0.5136 ns
hmgb EO (L. citrata) 0.5772 ns -
challenge 0.0055 **
Interaction 0.8219 ns
sod EO (L. citrata) 0.2664 ns -
challenge 0.6359 ns
Interaction 0.1313 ns
pxn EO (L. citrata) 0.0545 ns -
challenge 0.7649 ns
Interaction 0.9075 ns
tgase1 EO (L. citrata) 0.8293 ns -
challenge 0.2389 ns
Interaction 0.1047 ns
tgase2 EO (L. citrata) 0.1274 ns -
challenge 0.7944 ns
Interaction 0.1445 ns
12h lgbp EO (L. citrata) 0.2981 ns -
challenge 0.7485 ns
Interaction 0.8852 ns
dscam EO (L. citrata) 0.0045 ** -
challenge 0.0230 *
Interaction 0.1637 ns
hsp 70 EO (L. citrata) 0.0945 ns -
challenge 0.0241 *
Interaction 0.1816 ns
hmgb EO (L. citrata) 0.4481 ns antagonistic
challenge 0.9420 ns
Interaction 0.0263 *
sod EO (L. citrata) 0.5051 ns -
challenge 0.8230 ns
Interaction 0.1217 ns
pxn EO (L. citrata) 0.7266 ns -
challenge 0.0039 **
Interaction 0.7825 ns
tgase1 EO (L. citrata) 0.4951 ns -
challenge 0.1562 ns
Interaction 0.2472 ns
tgase2 EO (L. citrata) 0.2764 ns -
challenge 0.2137 ns
Interaction 0.4248 ns
24h lgbp EO (L. citrata) 0.4771 ns -
challenge 0.4263 ns
Interaction 0.8789 ns
dscam EO (L. citrata) 0.1625 ns -
challenge 0.3050 ns
Interaction 0.7909 ns
hsp 70 EO (L. citrata) 0.0109 * -
challenge 0.0077 **
Interaction 0.4646 ns
hmgb EO (L. citrata) 0.2385 ns -
challenge 0.4116 ns
Interaction 0.4688 ns
sod EO (L. citrata) 0.0014 ** -
challenge 0.7530 ns
Interaction 0.6294 ns
pxn EO (L. citrata) 0.0053 ** -
challenge 0.1603 ns
Interaction 0.1004 ns
tgase1 EO (L. citrata) 0.0005 *** -
challenge 0.0738 ns
Interaction 0.4440 ns
tgase2 EO (L. citrata) 0.1593 ns -
challenge 0.6452 ns
Interaction 0.5054 ns
C
Time point Gene Factors P value P value summary Interaction
6h lgbp EO (Eucalyptus citriodora) 0.2767 ns -
challenge 0.6524 ns
Interaction 0.8772 ns
dscam EO (E. citriodora) 0.0964 ns -
challenge 0.0066 **
Interaction 0.0705 ns
hsp 70 EO (E. citriodora) 0.7276 ns -
challenge 0.3050 ns
Interaction 0.8937 ns
hmgb EO (E. citriodora) 0.0694 ns antagonistic
challenge 0.1342 ns
Interaction 0.0042 **
sod EO (E. citriodora) 0.6201 ns antagonistic
challenge 0.3071 ns
Interaction 0.0130 *
pxn EO (E. citriodora) 0.8847 ns -
challenge 0.1854 ns
Interaction 0.2631 ns
tgase1 EO (E. citriodora) 0.5771 ns antagonistic
challenge 0.0188 *
Interaction 0.0082 **
tgase2 EO (E. citriodora) 0.0052 ** antagonistic
challenge 0.6609 ns
Interaction 0.0079 **
12h lgbp EO (E. citriodora) 0.0504 ns -
challenge 0.9434 ns
Interaction 0.7976 ns
dscam EO (E. citriodora) 0.0032 ** -
challenge 0.1783 ns
Interaction 0.8676 ns
hsp 70 EO (E. citriodora) 0.8764 ns -
challenge 0.5147 ns
Interaction 0.6000 ns
hmgb EO (Eucalyptus citriodora) 0.7198 ns -
challenge 0.0215 *
Interaction 0.6577 ns
sod EO (E. citriodora) 0.8301 ns -
challenge 0.0914 ns
Interaction 0.8695 ns
pxn EO (E. citriodora) 0.0408 * -
challenge 0.1499 ns
Interaction 0.5417 ns
tgase1 EO (E. citriodora) 0.9968 ns -
challenge 0.1144 ns
Interaction 0.2009 ns
tgase2 EO (E. citriodora) 0.7905 ns -
challenge 0.1167 ns
Interaction 0.3772 ns -
24h lgbp EO (E. citriodora) 0.1281 ns -
challenge 0.8545 ns
Interaction 0.7889 ns
dscam EO (E. citriodora) 0.0344 * -
challenge 0.0041 **
Interaction 0.1328 ns
hsp 70 EO (E. citriodora) 0.0567 ns -
challenge 0.0059 **
Interaction 0.1879 ns
hmgb EO (E. citriodora) 0.7132 ns antagonistic
challenge 0.8458 ns
Interaction 0.0250 *
sod EO (E. citriodora) 0.1185 ns -
challenge 0.4891 ns
Interaction 0.6296 ns
pxn EO (E. citriodora) 0.5817 ns antagonistic
challenge 0.0816 ns
Interaction 0.0292 *
tgase1 EO (E. citriodora) 0.0001 **** synergistic
challenge 0.0001 ****
Interaction 0.0010 **
tgase2 EO (E. citriodora) 0.1744 ns antagonistic
challenge 0.0125 *
Interaction 0.0096 **

The larvae were treated with either three different essential oils at an optimized concentration or challenged with/without V. campbellii at 107 cells/ml. Data represent the mean of four replicates. (Two-way ANOVA; ns, no significant differences; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; no interaction); EO, essential oil.