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. 2021 Oct 20;9:748804. doi: 10.3389/fcell.2021.748804

FIGURE 3.

FIGURE 3

The effect of microtubule (MT) stabilization in YAP expression in SMSCs. (A) Western blot analysis of phosphorylated Yes-associated protein (YAP), total YAP and TAZ expression in synovial mesenchymal stem cells (SMSCs) treated with nocodazole (N) and docetaxel (D) in chondrogenic medium (CM) for 1 week. (B) Quantification of the data of (A). n = 5. (C) Immunofluorescence staining for YAP of SMSCs. Scale bar, 100 μm. (D) Quantification of YAP in nucleus. n = 5. MT stabilization inhibited the YAP expression in SMSCs. (E) Co-immunofluorescence staining images of F-actin and YAP in SMSCs without docetaxel or nocodazole treatment (upper panel: view 1; lower panel: view 2). Scale bar, 200 μm. (F) RT-qPCR analysis of MST1, MST2, LATS1, LATS2, RHOA, ROCK1, and ROCK2 in SMSCs treated with either nocodazole (N) or docetaxel (D) for 1 week. Data are represented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.