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. 2021 Oct 20;9:748804. doi: 10.3389/fcell.2021.748804

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Copyright © 2021 Li, Sun, Lv, Jiang, Liu, Wang, Tan, Guo, Sun, Wu, Xu, Yan, Jiang, Ikegawa and Shi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The inhibition of microtubule (MT) stabilization in the formation of primary cilia in SMSCs. (A) Immunofluorescence staining of ARL13B (for representing primary cilia) in synovial mesenchymal stem cells (SMSCs) treated with nocodazole (N) and docetaxel (D) in chondrogenic medium (CM) for 1 week. (B) Quantification of (A) for the number and length of primary cilia in SMSCs. n = 6. (C) Western blot analysis of IFT88, SMO, and GLI1 in SMSC. (D) Quantification of the data of (C). n = 5. Data are represented as the mean ± SEM. **p < 0.01, ***p < 0.001.