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. 2021 Oct 20;9:748804. doi: 10.3389/fcell.2021.748804

FIGURE 5.

FIGURE 5

Knockdown of YAP promotes chondrogenesis and inhibits primary cilia formation in SMSCs. (A) Western blot analysis of Ace-Tubulin, phosphorylated Yes-associated protein (YAP), YAP, Col II, and SOX9 in synovial mesenchymal stem cells (SMSCs) transfected with siRNA-YAP and treated with docetaxel (D) for 1 week. (B) Quantification of the data of (A). n = 5. (C) Western blot analysis of IFT88, SMO, and GLI1 in SMSCs transfected with siRNA-YAP and treated with docetaxel (D) for 1 week. (D) Quantification of the data of (C). n = 5. (E) Immunofluorescence staining for ARL13B in SMSCs. (F) Quantification of (E) for the number and length of primary cilia in SMSCs. n = 5. Data are represented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.