FIGURE 2.

Effect of (A) pH and (B) temperature on the activity and stability of pLac _Gy . Unless otherwise stated, assay temperature was 60°C. Assay mixture included 0.5 µM pLac _Gy , 1 mM ABTS, 50 µM CuCl₂ in 0.04 M Britton-Robinson buffer (pH 5.0). For enzyme stability measurements, residual activity was measured at 60°C in presence of 1 mM ABTS and 50 μM CuCl₂ in 0.04 M Britton-Robinson buffer (pH 5.0) after pre-incubating the enzyme in the test condition for 6 h. The activity of the enzyme without pre-incubation measured at the pH 5.0 at 60°C was set at 100%. All experiments were conducted in triplicates and error bars represent standard deviations.